Figure 4.

Effect of intrauterine infusion on developmental capacity of oocytes following in vitro fertilization and embryo culture. Oocytes were collected via ultrasound-guided transvaginal oocyte pickup on days 2, 24, 45, and 66 relative to infusion of either LB broth vehicle medium (vehicle; n = 11) or pathogenic E. coli and T. pyogenes in LB broth (bacteria; n = 12) and subjected to in vitro fertilization and embryo culture. Pooled oocytes from each cow were maintained as an individual replicate throughout insemination and culture. Each dot represents an individual cow, and the solid line represents the mean of the treatment. The proportion of oocytes that cleaved 3.5 days post-insemination (A), the proportion of oocytes to develop to morulae 6 days post-insemination (B), and the proportion of cleaved oocytes to develop to morulae 6 days post-insemination (C) are shown. Comparisons between treatments on a specific day are indicated by ^* when P ≤ 0.05.