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. 2020 May 13;103(3):508–520. doi: 10.1093/biolre/ioaa069

Figure 5.

Figure 5

Effect of intrauterine infusion on gene expression of IVF derived morula stage embryos. Morula stage embryos derived by oocyte pickup, in vitro fertilization, and embryo culture from cows receiving intrauterine infusion of either LB broth vehicle medium (vehicle; n = 11) or pathogenic E. coli and T. pyogenes in LB broth (bacteria; n = 12) were probed for gene expression of BAX (A), BCL2 (B), DNMT3A (C), HSPA1A (D), IGF2R (E) and SLC2A1 (F) by real-time RT-PCR. Data are presented as expression relative to the geometric mean of the housekeeping genes GAPDH, SDHA, and RLP19. Each dot represents the average expression for an individual cow, and the solid line represents the mean of the treatment.