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. 2020 May 13;103(3):508–520. doi: 10.1093/biolre/ioaa069

Figure 8.

Figure 8

Effect of intrauterine infusion on gene expression of trophectoderm from in vivo-derived embryos. Cows were synchronized and inseminated on day 130 post infusion of either LB broth vehicle medium (vehicle; n = 11) or pathogenic E. coli and T. pyogenes in LB broth (bacteria; n = 12). Total RNA was isolated from trophectoderm of day 16 in vivo-derived embryos, and expression of CDKN1C (A), IFNT2 (B), and PPARG (C) was evaluated by real-time RT-PCR. Data displayed are expression relative to the geometric mean of the housekeeping genes ACTB and GAPDH. Each dot represents an embryo and the solid line depicts the mean.