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. 2020 Aug 21;11:4205. doi: 10.1038/s41467-020-18020-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a OCR and ECAR were measured for each of BAY-876-sensitive lines (red) and resistant lines (black). b OCR and ECAR ratio were calculated for each cell line. Student’s t test, ****p < 0.0001. c ECAR values measurement of cells with or without BAY-876 treatment for 5 days. n = 4; mean ± s.d.; Two-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001. d ECAR values measurement of cells with siRNA of GLUT1 knockdown or luciferase control. n = 7; mean ± s.d. Two-way ANOVA. ****p < 0.0001. e Glucose uptake analysis were performed in cells following BAY-876 treatment for the 5 days. n = 4; mean ± s.d. Two-way ANOVA. ***p < 0.001; ****p < 0.0001; f A trace of OCR values from a mitochondrial stress test of cells with or without BAY-876 treatment for 5 days. n = 4; mean ± s.d. Two-way ANOVA. ****p < 0.0001; n.s. not significant. g A trace of OCR values from a mitochondrial stress test of cells with siRNA of GLUT1 or luciferase control. n = 6; mean ± s.d. Two-way ANOVA. ***p < 0.001; ****p < 0.0001; n.s. not significant. h Glutamine uptake analysis was performed in cells following 1 μM BAY-876 treatment. n = 3. i Glutamine uptake analysis performed in cells transfected with 25 nM siSLC2A1 (bottom). n = 2. j Growth curves of MDA-MB-468 cells (left) and MDA-MB-436 cells (right) cultured in complete DMEM medium with or without glutamine deprivation treated with indicated nine doses of BAY-876 for 5 days. n = 3; mean ± s.d. Two-way ANOVA. **p < 0.01; ***p < 0.001; ****p < 0.0001. Source data are provided as a Source Data file.