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. 2020 Aug 12;11(8):683. doi: 10.1038/s41419-020-02848-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Analysis of protein expression by western blot. B4G12 cells were transfected with the same amount of Flag-tagged PAX6 WT or PAX6 K53/89R plasmid. Cell lysates were proved with anti-Flag and anti-GAPDH antibodies. b Relative expression levels of PAX6 were calculated. Note: de-SUMOylation induced the increased PAX6 protein level. c B4G12 cells were transfected with either PAX6 WT or PAX6 K53/89R plasmid, and then treated with 20 μg/ml CHX for 0, 12, or 24 h as indicated inhibiting protein synthesis. Cell lysates were analyzed by western blot using anti-Flag and anti-GAPDH antibodies. d The ratio of PAX6 protein was compared. Error bars showed means ± SEM (n = 3/group; *p < 0.05).