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. Author manuscript; available in PMC: 2021 Sep 3.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Jul 30;529(4):1011–1017. doi: 10.1016/j.bbrc.2020.07.002

Figure 4. LY83583 treatment enhanced proline hydroxylation on Type I collagen.

Figure 4.

(a) Similar amount of affinity purified type I collagen from MEF cells culture medium was blotted with anti-hydroxyproline specific antibody (panel 1) or anti-Type I collagen specific antibody (panel 2). More hydroxyproline was detected from samples 6 hrs after addition of LY83583. (b) To assess the effect of LY83583 at 6 hrs on hydroxyproline content, protein from MEF cells culture medium was concentrated, separated by SDS-PAGE, probed with antibody to Type I collagen, and the blot stripped and re-probed with antibody specific for hydroxyproline. More hydroxyproline was detected after addition of LY83583. * indicated pepsin-resistant col 1 α2.