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. 2020 Aug 5;9(9):7. doi: 10.1167/tvst.9.9.7

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Figure 3. — DS-7080a attenuates HUVEC migration induced by conditioned medium from human retinal pigment epithelial cells. (A) The concentrations of 11 growth factors and nine chemokines in the conditioned medium derived from polarized HRPE cells were determined by multiplex immunoassay. To obtain the conditioned medium, HRPE cells were cultured on transwells for about 1 month to allow for cell polarization. Each column is represented by the mean concentration of triplicate wells. (B) Effects of DS-7080a (DS) and ranibizumab (Rani) on HUVEC migration stimulated with the HRPE-conditioned medium were evaluated. Total contents of antibodies and Fab fragments were adjusted by adding hIgG2 and hFab. Migrated HUVECs were measured after 3 hours of incubation. HUVEC migration is shown in relative fluorescent units (RFU). Each point represents the mean ± SE of sextuplicate wells.