Skip to main content
. 2020 Aug 21;3(11):e201900630. doi: 10.26508/lsa.201900630

Figure 1. In vivo characterization of ORC-Pch2.

Figure 1.

(A) Schematic of hexameric Pch2 AAA+ assembly, with domains organization of Pch2. (B) Co-immunoprecipitation (co-IP) of wild-type Pch2 and Pch2–E399Q with Orc1–TAP (via α-TAP-IP) during the meiotic prophase (5 h into meiotic program). (C) co-IP of wild-type Pch2 and Pch2–K320R with Orc1–TAP (via α-TAP-IP) during the meiotic prophase (5 h into meiotic program). (D) Schematic of the Orc1-6/AAA+ complex and its canonical role with the Cdc6 AAA+ protein (and additional factors) in MCM/AAA+ complex loading and DNA replication. (E) co-IP of Pch2–E399Q with Orc1–TAP, Orc2–TAP, and Orc5–TAP during the meiotic prophase (5 h into meiotic program) (via α-TAP-IP). For α-HA, short and long exposures are shown. * indicate degradation products of either Orc1–TAP or Orc2–TAP. (F) co-IP of Pch2–E399Q with Orc2 (via α-Orc2 IP). Isotype IgG IP was used as negative control.