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. 2020 Aug 21;3(11):e201900630. doi: 10.26508/lsa.201900630

Figure 2. In vitro reconstitution of the origin recognition complex (ORC)–Pch2 complex.

Figure 2.

(A) Size exclusion chromatography of His–MBP–Pch2 purified from insect cells. Coomassie Brilliant Blue (CBB) staining of peak fractions (dotted line) run on SDS–PAGE gel. * indicates likely monomeric fraction of His–MBP–Pch2. AU, arbitrary units. (B, C) Amylose-based pulldown of the ORC (His–Orc1-6 and His–ORC) purified from insect cells, with His–MBP–Pch2. (B) CBB staining, (C) Western blot analysis using α-MBP and α-ORC (which recognizes all six ORC subunits). (D) Size exclusion chromatography of His–ORC (His–MBP–Pch2) assembly. CBB staining of peak fractions (dotted line) run on SDS–PAGE gel. AU, arbitrary units.