Figure S1. In vivo analysis of Pch2–ORC interactions.

(A) Western blotting of yeast strains expressing Pch2 with Orc1–TAP, Orc2–TAP and Orc5–TAP. Time points after meiosis induction are shown. * denotes Orc1–TAP degradation fragment. (B) Co-immunoprecipitation of Pch2–E399Q with Orc1–TAP (via α-TAP-IP) during the meiotic prophase (5 h into meiotic program) in the presence or absence of Benzonase. (C) DNA PAGE analysis of (B).