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(A) Amylose-based pulldown of His–Hop1, with His–MBP–Pch2 or MBP–His; Coomassie Brilliant Blue staining. (B) Amylose-based pulldown of His–Hop1, with His–MBP–Pch2 or MBP–His, in the presence or absence of ATP or ATPγS; Coomassie Brilliant Blue staining.
