Figure S6. In vivo analysis of the role of Cdc6 in Pch2/ORC interaction and function.

(A) Flow cytometry analysis of CDC6 and cdc6-mn (pSCC1::CDC6) in meiosis. Time points after induction into the meiotic program are indicated. (B) Co-immunoprecipitation of Pch2–E399Q with the ORC (via α-HA-IP) during the meiotic prophase (5 h into meiotic program) in CDC6 and cdc6-mn background. (C) Southern blot analysis of the YLR164W locus (right ribosomal DNA flank; chromosome XII) and YCR047C locus (control double-strand break [DSB] region; chromosome III), in dmc1Δ, pch2Δ dmc1Δ, cdc6-mn dmc1Δ, and cdc6-mn pch2Δ dmc1Δ background. dmc1Δ is a DSB repair deficient mutant used to detect accumulation of meiotic DSBs. * indicates aspecific signal.