Figure 1. Purification of antimicrobial peptides from Capsicum chinense.
(A) Chromatogram of the F/0-70 fraction from C. chinense fruits in C18 reversed-phase column. The column was equilibrated and run with 0.1% TFA (Solvent A) and eluted using a linear gradient of 100% propanol in 0.1% TFA (Solvent B), flow 0.5 ml.min−1. (B) Electrophoretic visualization in a SDS-Tricine-gel of F/0-70 fraction from C. chinense fruits treated with β-mercaptoethanol; M, Molecular mass marker (kDa).