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. 2020 Aug 17;12:7305–7317. doi: 10.2147/CMAR.S259677

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© 2020 Yang et al.

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High concentrations of allicin reduce IE2 protein expression. U87MG cells transfected with IE2 plasmid were treated with a concentration gradient of allicin (10 µg/mL, 30 µg/mL, and 60 µg/mL). (A) Expression of IE2 protein levels in U87MG cells after treated with a concentration gradient of allicin. (B) The detection of IE2 mRNA in U87MG cells by RT-qPCR, *P<0.05 and **P<0.01 vs control. Data are presented as the mean ± standard deviation of three independent experiments.

Abbreviations: IE2, intermediate-early gene 2; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.