Figure 2.

Depletion of circ_0026359 decreased CDDP resistance of gastric cancer cells. CDDP-resistant gastric cancer cells SGC-7901/CDDP and MKN-45/CDDP were transfected with si-circ_0026359#1, si-circ_0026359#2, or siNC. (a) Expression levels of circ_0026359 were examined by RT-qPCR. GAPDH was used as control. (b, c) These cells were treated with 1 μg/ml CDDP for 24, 36, and 48 hours, and cell viabilities were examined by the MTT assay. (d) IC50 for CDDP in these cells were analyzed by the MTT assay. (e) Cell colony formation assay was performed in these cells with 1 μg/ml CDDP treated for 48 hours. (f) Caspase-3/7 assay, (g) DNA fragmentation assay, and (h) flow cytometry with Annexin V-FITC and PI staining were performed in these cells with 1 μg/ml CDDP treated for 48 hours. ^∗∗P < 0.01.