Skip to main content
. 2020 Aug 13;2020:5103272. doi: 10.1155/2020/5103272

Figure 4.

Figure 4

POLD4 was a direct target of miR-1200. (a) Predicted consequential pairing of miR-1200 with the POLD4 3′UTR and the mutant consequence of POLD4 3′UTR used in the luciferase reporter assay. (b, c) Luciferase reporter assay of SGC-7901/CDDP and MKN-45/CDDP cells cotransfected with miR-1200/miR-NC mimics and luciferase reporter plasmid pmirGLO containing wild-type POLD4 3′UTR sequence (hsa_POLD4 3′UTR-wt)/mutant POLD4 3′UTR sequence (hsa_POLD4 3′UTR-mt). (d) mRNA levels of POLD4 in SGC-7901/CDDP and MKN-45/CDDP cells after transfection with miR-1200/miR-NC mimics were examined by RT-qPCR. U6 was detected as control. (e) Protein levels of POLD4 in SGC-7901/CDDP and MKN-45/CDDP cells after transfection with miR-1200/miR-NC mimics were examined by western blot. α-Tubulin was detected as control. ∗P < 0.05; ∗∗P < 0.01. ns: no significance.