Table 6.

Stability of the components in rat plasma sample.

Compounds	Concentration (ng/ml)	Room temperature (3 h, 25°C)		Autosampler (24 h, 4°C)		Three freeze/thaw cycles		Long term (30 day, −80°C)
		Precision (RSD%)	Accuracy (RE%)	Precision (RSD%)	Accuracy (RE%)	Precision (RSD%)	Accuracy (RE%)	Precision (RSD%)	Accuracy (RE%)
Stachydrine	10.00	4.62	-9.23	2.66	-9.94	4.44	-7.37	4.69	-5.94
	250.00	0.81	0.09	4.06	-6.0	2.59	6.17	3.49	2.03
	2000.00	4.49	3.14	3.82	3.07	3.55	5.27	3.37	4.64
Danshensu	2.00	5.29	7.09	5.29	7.09	6.92	-2.70	8.08	6.79
	50.00	10.89	-10.20	10.89	-10.20	1.58	-12.52	5.55	-4.84
	400.00	2.78	4.73	2.78	4.73	0.82	0.91	1.22	2.60
Chlorogenic acid	0.20	6.13	-3.40	1.98	-1.57	5.29	-3.89	4.57	-0.48
	5.00	0.45	11.70	0.80	13.89	4.07	9.84	1.25	10.93
	40.00	0.66	11.51	0.65	14.17	1.20	13.15	0.53	12.59
Protocatechuic acid	0.20	3.69	7.78	1.00	12.84	1.60	1.80	3.87	5.18
	5.00	2.55	10.39	1.99	5.52	1.03	8.49	3.58	8.11
	40.00	0.95	10.48	1.97	8.98	2.29	8.55	0.50	7.28
Plantamajoside	0.10	2.00	9.23	5.21	7.94	2.91	6.68	7.05	4.14
	2.50	1.28	-90.31	5.08	-91.35	2.59	-91.11	3.79	-90.84
	20.00	1.36	5.03	1.94	-0.13%	2.87	-5.26	1.20	-0.43
Aesculetin	0.20	5.16	-1.16	1.51	4.88	3.02	2.69	4.20	-0.62
	5.00	2.41	7.25	0.89	8.31	1.95	9.94	1.79	6.76
	40.00	2.07	11.25	4.89	9.87	1.90	7.39	3.61	7.41
Isoquercitrin	0.20	2.65	3.57	2.55	4.81	2.56	8.27	1.88	0.43
	5.00	0.66	5.18	0.48	5.40	1.58	4.20	0.51	5.56
	40.00	0.54	11.02	2.16	11.09	2.43	10.30	0.63	8.27
Ferulic acid	2.00	3.09	5.81%	11.12	6.79	7.50	4.93	4.72	7.22
	50.00	2.37	4.96	0.67	4.55	1.47	6.41	0.99	5.32
	400.00	0.38	3.57	2.20	4.12	1.39	4.29	0.06	2.22
Baicalin	10.00	1.38	-6.39	4.18	-5.56	5.63	-0.46	4.01	-6.58
	250.00	0.37	-10.18	1.38	-11.90	2.72	-0.02	4.76	-5.18
	2000.00	7.12	-3.62	4.10	-6.54	5.25	-7.42	3.31	-5.38
Baicalein	2.00	6.37	-3.82	2.29	-7.65	1.69	-3.44	2.30	-10.32
	50.00	2.29	-9.87	2.99	-8.08	3.72	-1.39	4.60	-5.23
	400.00	5.15	0.51	4.11	-5.84	3.38	-0.69	3.42	-8.34