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. Author manuscript; available in PMC: 2020 Aug 23.
Published in final edited form as: Cell Rep. 2020 Apr 14;31(2):107500. doi: 10.1016/j.celrep.2020.03.064

Figure 1. Immunohistochemical Analysis Reveals Diffuse Infiltration of the Cortex and Progressive Disruption of the Vasculature within the Highly Cellular Core of the Tumor.

Figure 1.

(A) Coronal sections obtained from glioma-bearing mice at 22 and 28 days post injection (DPI) show the distribution of glioma cells (HA; red), GCaMP6f+ neurons (green), and IB4-labeled vessels (magenta). Stitched images show a single confocal plane. Bar, 1,000 μm.

(B) 3D rendering of GCaMP6f+ neurons (green) surrounded by infiltrating glioma cells (red) (80 × 80 × 15 μm XYZ volume).

(C) Infiltrating glioma cells (red) migrate along blood vessels (arrowheads), with AQP4 (white) remaining closely associated with IB4+ vessels (magenta). Maximum intensity projections of confocal stacks. Bar, 50 μm.

(D) Representative fields of glioma at the indicated time points and locations. AQP4 association with IB4+ vessels is intact in the contralateral (uninfiltrated) cortex and at the infiltrative margins of the tumor, but is disrupted in the tumor core. Bar, 100 μm.

(E) Contralateral cortex and tumor core of a 22- and a 28-DPI tumor stained with CD34. Maximum intensity projections of confocal stack. Bar, 100 μm. Graphs (mean ± standard deviation) show fold change of CD34 signal intensity and area occupied in tumor versus contralateral cortex. C, contralateral cortex; T, tumor core. Two-tailed, unpaired t tests were used to calculate significance.