Figure 4. Spatial Variation of Initiation and Propagation of Interictal Events (Mouse 1).
(A) Raw fluorescence image marked with ROIs.
(B) ΔF/F image time-series of 3 epochs from a 180-s WFOM recording (without external stimulus) containing 13 spatially distinct interictal events (collected at DPI 35). Magenta arrows indicate areas of interictal event initiation.
(C) Time-courses of GCaMP6f fluorescence, HbO, HbR, and HbT extracted from the 5 ROIs in (A). The lower amplitude and altered hemodynamic responses in the tumor region (i) are clearly visible, as well as differing relative amplitudes of the IIEs in each region for each event, reflecting local or global spread.
(D) Contour plots showing the onset time to 20% of the maximum for each of the 13 events (a–m) denoted in (C). Dark red regions correspond to t = 0 initiation locations (see STAR Methods).
(E) Frequency of events for each mouse in each imaging session (all identified events, divided by the total duration of imaging). There is a significant increase in relative frequency of events over time (R = 0.3282, p value = 0.036177).