Fig. 8.

Effects of swainsonine treatment on tumor cell–selectin interaction. Pharmacologic inhibition of N-glycosylation in the Golgi using swainsonine had largely no effect on sLeA/X expression and static selectin binding except sLeX expression on GC5023 cells (A), static hESel binding by Molm13 cells and static mESel binding by HOS and SKOV3 cells (B). Effects on the dynamic adhesion on selectins varied among the cell lines as shown in (C–E). In particular, adhesions of HT29 cells on hESel and mPSel were decreased (C and E) while adhesions of SKOV3 cells on hESel, of Molm13 cells on mESel (C) and rolling of EOL-1 cells on hPSel were increased (D). Please see legend to Figure 3 for technical information. This figure is available in black and white in print and in colour at Glycobiology online.