Figure 3.

Significantly reduced testis weight, sperm number, motility, and abnormally formed sperm in the conditional Cops5 knockout mice. A. Representative Western blot result showing only a trace amount of COPS5 protein was expressed in the testes of Cops5 conditional knockout mice (n = 3). B. Significantly reduced testis size in the Cops5 knockout mice. Left: representative image of testes from three control and three conditional Cops5 knockout mice. Right: mean testis/body weight of the control and knockout mice, showing a statistically significant reduction in weight (^*), P < 0.05. C. Representative images of epididymal sperm from a control mouse and a conditional Cops5 knockout mouse. D. Significantly increased percentage of abnormal sperm in the Cops5 mutant mice (n = 10). E, F. Percentage of motile sperm and sperm motility was reduced in the conditional Cops5 knockout mice (n = 10). Statistically significant differences (^*); P < 0.05.