Table 1.
Antibody information.
Name | Catalogue number | Purpose | Dilution |
---|---|---|---|
Anti-COPS5/JAB1 | J3020, SIGMA | Western blot | 1:2000 for western blot; 1:200 for IF |
β-Actin | 4967 S, Cell Signaling Technology | Western blot | 1:2000 |
PARP | #9542, Cell Signaling Technology | Western blot | 1:2000 |
Caspase-3 | #9665, Cell Signaling Technology | Western blot | 1:2000 |
FANK1 | SC-398057, Santa Cruz Biotechnology | Western blot | 1:1000 |
SPACA1 | Dr. Oko’s Laboratory | Western blot | 1:4000 |
GAPDH | SC-32233, Santa Cruz Biotechnology | Western blot | 1:2000 |
Anti-IFT20 | Dr. G.J.P.’s Laboratory | Western blot | 1:2000 for western blot; 1:200 for IF |
Anti-α-tubulin | T9026-2ML, SIGMA | IF | 1:200 |
Anti-SOX9 | ab185966, Abcam | IF | 1:200 |
Anti-rabbit IgG | 711166152, Jackson ImmunoResearch | IF | 1:200 |
Anti-mouse IgG | DI-2488, Vector Laboratories | IF | 1:400 |