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. 2020 Aug 19;13:8233–8243. doi: 10.2147/OTT.S243040

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© 2020 Wang et al.

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Overexpression of circEXOC6B induced the apoptosis of ovarian cancer cells. SKOV3 cells were treated with circEXOC6B-shRNA2 or/and miR-421 antagonist for 72 h. (A, B) Apoptotic cells were detected with Annexin V and PI double staining. (C) Expression levels of Bcl-2 and cleaved caspase 3 in SKOV3 cells were detected with Western blotting. (D, E) The relative expressions of Bcl-2 and cleaved caspase 3 in SKOV3 cells were quantified via normalization to β-actin. **P < 0.01.