FIGURE 4.

Detection of human tissue factor by western blotting using commercial antibodies. We used a tissue factor (TF)‐positive cell line (HPAF‐II) and a TF‐negative cell line (MIA PaCa‐2). Each primary antibody was diluted 1:1000. The final concentration of each antibody was as follows: Novus Biologicals (final concentration 0.64 μg/mL); Cell Signaling Technologies (final concentration 0.055 μg/mL), HTF‐1 (final concentration 0.55 μg/mL), in‐house TF9‐10H10 (final concentration 1.0 μg/mL), Bio‐Rad Laboratories TF9‐10H10 (final concentration 0.055 μg/mL). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was detected using an anti‐human GAPDH antibody (1:1000 dilution, final concentration 0.2 μg/mL). Secondary antibodies were used at a 1:10 000 dilution in blocking buffer. A protein marker is shown on the left of each panel