FIGURE 5.

Detection of mouse tissue factor by western blotting using commercial antibodies. We used a tissue factor (TF)‐positive cell line (KPC2 Cas9) and a TF‐negative cell line (KPC2 TF KO). Each primary antibody was diluted 1:1000. The final concentration of each antibody was as follows: R&D Systems (final concentration 0.2 μg/mL), Abcam ab189483 (final concentration 0.576 μg/mL), BioMedica Diagnostics (final concentration 1.0 μg/mL), Abcam ab151748 (final concentration 0.212 μg/mL), and Abcam ab104513 (final concentration 0.55 μg/mL). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was detected using an anti‐mouse GAPDH antibody (1:1000 dilution, final concentration 0.2 μg/mL). Secondary antibodies were used at a 1:10 000 dilution in blocking buffer. A protein marker is shown on the left of each panel. The asterisk marks a nonspecific band. All data was generated with blots run in parallel and probed in parallel except the data for ab151748 was obtained on a different day using different samples