FIGURE 8.

Nitrobenzoyl sesquiterpenoid 4 (NS4) prevents inflammatory bone loss induced by LPS in vivo. Groups of mice injected with PBS (n = 6) or LPS (n = 6) but without treatment with NS4 or injected with LPS and treated with 1 mg·kg⁻¹ (n = 6) or 5 mg·kg⁻¹ NS4 (n = 6) were used. Representative 3D reconstructions of transverse (a, above) and longitudinal (a, below) sections of femur from each group by micro‐CT were shown. The parameters of trabecular bone including BMD, BV/TV, Tb. Sp, Tb. N, Cor. Th, Tb. Th and ConnD (b) were analysed. Sections of femur from each group were stained with H&E (c, above) and TRAP (c, middle and below). BV/TV (d), number of osteoclasts (e), and osteoclast surface/bone surface (f) were analysed. In addition, three groups (n = 5) of mice were treated with PBS, LPS and NS4 (5 mg·kg⁻¹) as described above and i.p. injected calcein 7 and 3 days prior to killing. Bone formation indicated by calcein staining was examined by fluorescence microscopy (g) and MAR was calculated (h). ^# P < 0.05 relative to PBS treated controls, *P < 0.05 relative to LPS treated controls