Table 1.

Thermodynamic parameters of manno-oligosaccharide binding to horcolin at 25 °C

n is stoichiometry coefficient (number of sugars bound/mol of horcolin monomer), ΔH is enthalpy, ΔS is entropy, and ΔG is reaction energy (calculated with the formula, ΔG = −RT ln K_b, where r = 1.987 cal/mol·K).

Ligand	n	Ka	KD	ΔH	ΔG	TΔS
		m−1		kcal/mol	kcal/mol	kcal/mol
Me-α-mannopyranoside	1.72 + 0.328	200 + 27.8	5.00 mm	−6.324	−3.1354	−3.1886
α-d-Mannose	1.90 + 0.521	101 + 12.5	9.90 mm	−5.517	−2.73368	−2.78332
Manα1–2-Man	1.946 + 0.4028	398.9 + 41.42	2.50 mm	−8.974	−3.5504	−5.4236
Manα1–3-Man	1.88 ± 0.0724	596 ± 14.6	1.67 mm	−10.46	−3.7848	−6.6752
Manα1–6-Man	1.78 ± 0.243	227 ± 10.3	4.40 mm	−14.88	−3.2282	−11.6518
Manα1–4-Man	1.77 ± 0.649	196 ± 21.2	5.10 mm	−10.28	−3.128	−7.152
Man3	2.01 + 0.0275	6.10 E3 + 300	163 μm	−12.94	−5.1622	−7.7778
Man5	0.774 ± 0.076	5.99 E4 ± 1.00 E5	16.69 μm	−16.76	−6.5088	−10.2512
Man7-D1	0.925 ± 0.0117	2.61 E4 ± 2.87 E4	3.831 μm	−17.1	−7.3256	−9.7744
Man7-D3	1.09 ± 0.00711	2.4 E5 ± 1.45 E4	4.16 μm	−15.99	−7.348	−8.642
Man9	1.34 + 0.0185	8.60 E4 + 1.21 E4	11.6 μm	−8.277	−6.73038	−1.54662