Table 3.
Oligonucleotides used to construct plasmids in this studya
| Oligonucleotide and mutation | Sequence (5'→3') | Source |
|---|---|---|
| YCK3 FP | CGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGtctgttccgtggcaaatgtttctcc | This study |
| YCK3 RP | CAAAAGCTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATttacatattattttggatattcttaaaaaaatgc | This study |
| YCK3-HAc FP | CTACTTTTTACAACAAATCTAGAATTCCTGCAGCCCGGGGGATCCatgtcccaacgatcttcacaacac | This study |
| YCK3-HAc RP | CCCGCATAGTCAGGAACATCGTATGGGTAAAAGATGCGGCCCAGATCacagcaacaaaaacagcaacaac-3' | This study |
| HAn-YCK3 FP | CGCTGCTCAGTGCGGCCCAGATCTGTCCCAACGATCTTCACAACACATTGTAGG | This study |
| HAn-YCK3 RP | TAGTACATAGGAAATTTTAAACGGTATACAAGTACGTAAAAAAGGTCAACAGCAACAAAAACAGCAACAAC | This study |
|
Truncated Env7 (1-304) FMP |
gttcggtatctccccctttgagcgataagagcagatacatggagcttctttaacc | This study |
|
Truncated Env7 (1–304) RMP |
ggttaaagaagctccatgtatctgctcttatcgctcaaagggggagataccgaac | This study |
| Env7-S331A FMP | gagaaattccagatttgctgaggggcttttgag | This study |
| Env7-S331A RMP | ctcaaaagcccctcagcaaatctggaatttctc | This study |
| Env7-S323A-S328A-S331A FMP | cctacgctataaacactggtaagtacgctttcccgagaaatgccagatttgctgaggggcttttgagtgt | This study |
| Env7- S323A-S328A-S331A RMP | acactcaaaagcccctcagcaaatctggcatttctcgggaaagcgtacttaccagtgtttatagcgtagg | This study |
| Env7-T351A-T352A-S353A FMP | gcattcaagtggatcctatacaaaggcctgctgccgcccaattattaaatcttttacaagatttagac | This study |
| Env7- T351A-T352A-S353A RMP | gcttaaatcttgtaaaagatttaataattgggcggcagcaggcctttgtataggatccacttgaatgc | This study |
| Env7-5×-A323S FMP | cctacgctataaacactggtaagtacagtttcccgagaaatgccagattttctgagg | This study |
| Env7-5×-A323S RMP | cctcagcaaatctggcatttctcgggaaactgtacttaccagtgtttatagcgtagg | This study |
| Env7-5×-A328S FMP | ggtaagtacgctttcccgagaaattccagatttgctgaggggcttttgag | This study |
| Env7-5×-A328S RMP | ctcaaaagcccctcagcaaatctggaatttctcgggaaagcgtacttacc | This study |
| Env7-5×-A331S FMP | cgctttcccgagaaatgccagattttctgaggggcttttgagtgtaatcaag | This study |
| Env7-5×-A331S RMP | cttgattacactcaaaagcccctcagaaaatctggcatttctcgggaaagcg | This study |
| Env7-5×-A353S FMP | ggatcctatacaaaggcctgctgccagccaattattaaatcttttacaag | This study |
| Env7-5×-A353S RMP | cttgtaaaagatttaataattggctggcagcaggcctttgtataggatcc | This study |
| Env7-S331D FMP | cctacgctataaacactggtaagtacagtttcccgagaaattccagatttgatgaggggcttttgagtg | This study |
| Env7-S331D RMP | cactcaaaagcccctcatcaaatctggaatttctcgggaaactgtacttaccagtgtttatagcgtagg | This study |
| Env7-5×-A331D FMP | cctacgctataaacactggtaagtacgctttcccgagaaatgccagatttgatgaggggcttttgagtgt | This study |
| Env7-5×-A331D RMP | acactcaaaagcccctcagcaaatctggcatttctcgggaaagcgtacttaccagtgtttatagcgtagg | This study |
| Env7-S323A-S331A FMP | cctacgctataaacactggtaagtacgctttcccgagaaattccagatttgctgaggggcttttgagtgt | This study |
| Env7-S323A-S331A RMP | acactcaaaagcccctcagcaaatctggaatttctcgggaaagcgtacttaccagtgtttatagcgtagg | This study |
a Vector-specific sequences are capitalized, and ENV7-specific sequences are in lowercase. Mutations are indicated in boldface, and affected codons are underlined. FP, forward primer; RP, reverse primer; FMP, forward mutant primer; RMP, reverse mutant primer.