Figure 2.
P. protegens forms Pel polysaccharide–dependent biofilms that are enhanced by the overexpression of the diguanylate cyclase, WspR. A and B, biofilm biomass determined using the crystal violet assay for (A) WT P. protegens Pf-5 and the corresponding deletion mutants assayed after 5 days of growth and (B) strains expressing pPSV39 (empty vector) or pPSV39::wspRR242A after 24 h of growth. Error bars indicate the S.E. of the mean of two (A) or three (B) independent trials performed in triplicate. Statistical significance was evaluated using one-way analysis of variance with Bonferroni correction. ***, p < 0.001; ****, p < 0.0001.