Figure 5.

PelX is a member of the short-chain dehydrogenase/reductase superfamily of enzymes. Cartoon representations of PelX^C232S. A, PelX^C232S is displayed as found in the asymmetric unit with its N-terminal Rossmann fold domain shown in green, and its C-terminal substrate-binding α/β-domain in purple. B, overlay of PelX^{C232S/Y146F/S121A} substrate complexes with the WT PelX^C232S colored according to the legend in the figure. C, comparison of the active site of PelX^{C232S/Y146F/S121A}–UDP-GalNAc complex, and WbpP–UDP-GalNAc complex (PDB ID: 1SB8). UDP-GalNAc and NADH (NAD⁺), and active site residues are shown in stick representation.