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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Bioorg Med Chem. 2020 Jul 17;28(18):115644. doi: 10.1016/j.bmc.2020.115644

Table 2.

IC50 values for Berberine chloride and Zinc chloride obtained using different constructs and expression systems

IC50 (μM)
Expression construct Bacterial Lysate TIR domaina Bacterial Lysate SAM1-2TIRb Expi293 Lysate SAM1-2TIRc Pure TIR domaind
Berberine Chloride 140 ± 20 110 ± 10 77 ± 5 140 ± 30
Zinc Chloride 10 ± 3 N.D. N.D. 10 ± 1
a

Experiments were performed with bacterially expressed TIR domain (300 nM) lysates and 50 μM ENAD.

b

Experiments were performed with bacterially expressed SAM1-2TIR domain (300nM) lysates and 50 μM ENAD.

c

Experiments were performed with mammalian Expi293 lysates expressing SAM1-2TIR domain (300 nM) and 50 μM ENAD.

d

Experiments were performed with purified TIR domain (20 μM) and 500 μM ENAD.