Table 4:
Key Resources1.
| Reagent or Resource | Source | Identifier | |
|---|---|---|---|
| 4-Chloronicotinamide | Matrix Scientific | 7418-70-4 | |
| 96-well Half Area Black Flat Bottom Polystrene NBS™ plate | Corning® | 3993 | |
| ADPR | Sigma Aldrich | A0752 | |
| ADPR cyclase | Sigma Aldrich | A9106, C7344 | |
| Berberine chloride | Sigma Aldrich | B-3251; PHR1502 | |
| C43 (DE3) | Sigma Aldrich | CMC0019 | |
| cADPR | Sigma Aldrich | C7344 | |
| Doxycycline hydrochloride | Sigma Aldrich | D3447 | |
| Kanamycin | Research Products International | 25389-94-0 | |
| Lysozyme | Sigma Aldrich | L6876 | |
| Nicotinamide | Sigma Aldrich | 72340 | |
| Nicotinamide 1,N6-ethenoadenine dinucleotide | Sigma Aldrich | N2630 | |
| Nitroflurazone | Sigma Aldrich | PHR1196 | |
| Phenazopyridine hydrochloride | Sigma Aldrich | 34076 | |
| Pierce™ EDTA-free protease inhibitor tablets | Thermo Fisher Scientific | A32955 | |
| Pyrithone zinc | Sigma Aldrich | PHR1401 | |
| Streptacin XT | IB A Life Sciences | 2-4010-025 | |
| Streptavidin Antibody | LI-COR Biosciences IR Dye 680RD | 976-68079 Lot #C60504-02 | |
| Talon Metal Affinity Resin | Takara | #635503 | |
| Halt Protease Inhibitor Cocktail | Thermo Fisher Scientific | 78429 | |
| Constructs | |||
| pET30a+ Strep-SAM1-2 TIR | 15 | ||
| pET30a+ Strep-TIR | 15 | ||
| Mammalian Flag-SAM1-2TIR For | IDT | CAGGATCCACCATGGACTACAAGGACGACGACGACAAGTGGAAGGAGGCCGAGGTTCAGACGTGGCTG | |
| Mammalian Flag-SAM1-2TIR Rev | IDT | TTGGAGGGTGCTGCACCCATGGGTCCAACCTAAACCCTCGAGCA | |
| C629A For | IDT | GCGCTGGATAAGGCTATGCAGGACCACGAT | |
| C629A Rev | IDT | CGCGACCTATTCCGATACGTCCTGGTGCTA | |
| C635A For | IDT | ATGCAGGACCACGATGCGAAGGACTGGGTG | |
| C635A Rev | IDT | TACGTCCTGGTGCTACGCTTCCTGACCCAC | |
1
See Table 2 from 15