Supplemental Data Table S2.
Analyte and internal standard MS parameters
| Analyte | Transition | MRM transition (m/z) | Collision energy (V) | Cell accelerator voltage (V) | RT (min) |
|---|---|---|---|---|---|
| Melatonin | Quantifier | 233.1 → 174.1 | 9 | 4 | 1.3 |
| Qualifier | 233.1 → 159.2 | 33 | 4 | 1.3 | |
|
| |||||
| Melatonin-d4 | Quantifier | 237.1 → 178.1 | 17 | 4 | 1.3 |
|
| |||||
| Cortisol | Quantifier | 363.1 → 120.9 | 25 | 4 | 2.2 |
| Qualifier | 363.1 → 327.4 | 13 | 4 | 2.2 | |
|
| |||||
| Cortisol-d4 | Quantifier | 367.4 → 121.1 | 25 | 4 | 2.2 |
Abbreviations: MS, mass spectrometry; MRM, multiple reaction monitoring; RT, retention time.