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. 2020 Aug 11;8:767. doi: 10.3389/fcell.2020.00767

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Copyright © 2020 Gesper, Wennmalm, Hagemann, Eriksson, Happel and Parmryd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of curved membrane sections on FCS recordings. (A) The most common model for analyzing diffusion in two dimensions with FCS assumes a locally flat membrane aligned perpendicular to the optical axis of the excitation beam (green elongated spot). Gray arrows indicate the length of the path from one side of the excitation spot to the other. (B) If the membrane is locally curved, the path becomes longer (indicated by the additional black arrows) due to the surplus of membrane within the excitation spot, which leads to longer transit times determined by FCS.