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. 2020 Aug 11;8:767. doi: 10.3389/fcell.2020.00767

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Copyright © 2020 Gesper, Wennmalm, Hagemann, Eriksson, Happel and Parmryd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modeling spot size variation FCS reports topography-induced apparent anomalous diffusion. (A) Mean transit times (±SD, n = 5) for varying spot sizes. Spot sizes are presented as multiples of the area of the reference spot size (FWHM of ∼200 nm) used in Figure 5C. (B) Scatter plot of the parameter α obtained from fitting the model A × s^α to the data in panel (A) and the transit time at the reference spot size. The Pearson correlation coefficient for the two variables was −0.70 (p = 0.0044).