FIGURE 2.

BRG1 regulates NO bioavailability by modulating eNOS phosphorylation in cultured endothelial cells. (A–C) EAhy926 cells and HAECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with oxLDL (10 μg/ml) for 24 h. NO content in the culture media was examined by the Griess assay. Gene expression levels were examined by qPCR and Western. (D–F) EAhy926 cells and HAECs were treated with oxLDL (10 μg/ml) in the presence or absence of PFI-3 (5 μM) for 24 h. NO content in the culture media was examined by the Griess assay. Gene expression levels were examined by qPCR and Western. *p < 0.05.