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. 2020 Aug 12;8:774. doi: 10.3389/fcell.2020.00774

FIGURE 3.

FIGURE 3

BRG1 mediates PP2A transcription. (A,B) 8-week male Apoe–/– mice were injected with lentivirus carrying endothelial-specific BRG1 shRNA (Endo-shBRG1) or the control virus (shC) and fed a Western diet or a control diet for 8 weeks as described in “Materials and Methods”. Expression levels of specific genes in the aortic arteries were examined by qPCR and Western. N = 5 mice for the control diet groups and N = 7 mice for the Western diet groups. (C,D) EAhy926 cells and HAECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with oxLDL (10 μg/ml) for 24 h. Gene expression levels were examined by qPCR and Western. (E,F) EAhy926 cells and HAECs were treated with oxLDL (10 μg/ml) in the presence or absence of PFI-3 (5 μM) for 24 h. Gene expression levels were examined by qPCR and Western. *p < 0.05.