FIGURE 4.

PP2A over-expression circumvents BRG1 deficiency to suppress NO production. (A,B) EAhy296 cells were transfected with siRNA targeting BRG1 in the presence or absence of exogenous HA-tagged PR65α followed by treatment with oxLDL (10 μg/ml) for 24 h. NO content in the culture media was examined by the Griess assay. eNOS phosphorylation was examined by Western. (C,D) EAhy296 cells were transfected with exogenous HA-tagged PR65α followed by treatment with oxLDL (10 μg/ml) and PFI-3 (5 μM) for 24 h. NO content in the culture media was examined by the Griess assay. eNOS phosphorylation was examined by Western. *p < 0.05.