FIGURE 5.

BRG1 interacts with ETS1 to regulate PR65A transcription. (A) PR65A promoter-luciferase constructs were transfected into EAhy926 cells with or without BRG1 followed by treatment with oxLDL (10 μg/ml). Luciferase activities were normalized by protein concentration and GFP fluorescence. (B) EAhy926 cells and HAECs were treated with oxLDL (10 μg/ml) and harvested at indicated time points. ChIP assays were performed with anti-BRG1 or IgG. (C) Wild type and mutant PR65A promoter-luciferase constructs were transfected into EAhy926 cells with or without BRG1 followed by treatment with oxLDL (10 μg/ml). Luciferase activities were normalized by protein concentration and GFP fluorescence. (D) FLAG-tagged BRG1 and V5-tagged EST1 were transfected into HEK293 cells. Immunoprecipitation was performed with anti-FLAG. (E) Nuclear lysates extracted from EAhy926 cells were immunoprecipitated with anti-BRG1. (F) EAhy926 cells and HAECs were with oxLDL (10 μg/ml) for 24 h. Re-ChIP assays were performed with indicated antibodies. *p < 0.05.