Skip to main content
. 2020 Aug 13;10:1612. doi: 10.3389/fonc.2020.01612

FIGURE 1.

FIGURE 1

CircHIPK3 was upregulated by HIF-2α in HRGC cells. (A,B) The migration and invasion ability of HRGC cells and their parent GC cells was examined by transwell assay (original magnification, 100×). The columns on the right are quantified by counting three fields, and presented as the mean ± standard deviation. *p < 0.05, **p < 0.01. (C) The protein expression of HIF-1α and HIF-2α in HRGC cells compared with their parent GC cells was detected by western blot. β-actin was used as an internal control. (D,E) The relative expression of circHIPK3 and linear HIPK3 mRNA in HRGC cells and their parent GC cells was detected by qRT-PCR. (F,G) The knockdown efficiency of HIF-1α or HIF-2α in HRGC cells was detected by qRT-PCR. (H,I) The relative expression of circHIPK3 in HRGC cells after transfected with HIF-1α or HIF-2α siRNAs was detected by qRT-PCR. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. 18S was used as an internal control for all qRT-PCR experiments.