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. 2020 Aug 13;10:1612. doi: 10.3389/fonc.2020.01612

FIGURE 5.

FIGURE 5

CircHIPK3 promoted migration and invasion of HRGC cells via the NRP1-ERK/AKT pathway. (A) The knockdown efficiency of NRP1 in HRGC cells was detected by western blot. (B,C) The migration and invasion ability of HRGC cells after transfected with siNC or NRP1 siRNAs was examined by transwell assay (original magnification, 100×). The columns on the down panels are quantified by counting 3 fields, and presented as the mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001. (D) The downstream pathway proteins in HRGC cells after transfected with siNC or NRP1 siRNAs were detected by western blot. (E) The same downstream pathway proteins as (D) in HRGC cells after transfected with siNC or circHIPK3 siRNAs were detected by western blot. (F) The relative expression of NRP1 in GC tissues and adjacent normal tissues was analyzed by GEPIA database. (G) The overall survival of GC patients with NRP1-high expression or NRP1-low expression was analyzed by GEPIA, Kaplan-Meier Plotter and TCGA databases. Data are presented as the mean ± SD of three independent experiments. β-actin was used as an internal control for all western blot assays.