Figure 5.
The Lateral Accessory Calyx Is a Thermo- and Hygrosensory Hub with Outputs to Multiple Neuropils
(A–K, except D’’) Frontal views of reconstructions (black) of non-KC lACA target neurons. Brain neuropils have been color-coded to correspond with all other figure panels. (A) All identified targets with at least five connections to lACA PNs within the lACA. (B–K) Strong downstream targets of lACA PNs. Presynaptic sites in red and postsynaptic sites in cyan. (B) LHAV3q1#1, (C) AV3r1#1, (D) DN1a#1, (D') DN1a#2, (E) DN1-l, (F) LHPV4c1#1, (F') LHPV4c1#2, (G) LHAV3f1#1, (H) PD5g1#1, (I) LHPD5a1#1, (J) LHAV2d1#1, (K) PV6r1#1. AME: accessory medulla.
(D’’) Gal4 driver line labeling DN1a neurons (source: Fly Light).
(L–M) Circuit diagrams depicting (L) within-lACA and (M) total connectivity between lACA PNs and their strong downstream targets. Letters correspond to those of panels in this figure (except for elements carried over from Figure 3 and dark red VP2: VP2+ adPN). Circular nodes represent individual neurons; hexagonal nodes represent pooled neurons, with the number pooled after the hashtag. Synapse numbers correlate with line thicknesses, with dashed arrows in (M) representing inputs from (L) of <10 connections. CA PNs: olfactory PNs (O) and VP PNs (VP) targeting KC dendrites in the main calyx. lACA output neurons: neurons receiving input from lACA PNs that have <10 outputs to other lACA circuit neurons.