REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-nc82 (1:200)	DSHB, University of Iowa	Bruchpilot; RRID:AB_2314866
chicken anti-GFP (1:100)	Avēs Labs Inc.	Cat#: GFP-1010; RRID:AB_10000240
rabbit anti-DsRED (1:200)	Takara Bio	Cat#: 632392; RRID:AB_10013483
goat anti-mouse Cy5 (1:200)	Jackson ImmunoResearch Labs	Cat#: 115-175-166; RRID:AB_2338714
goat anti-chicken 488 (1:200)	Invitrogen	Cat#: A-11039; RRID:AB_142924
goat anti-rabbit Cy3 (1:200)	Jackson ImmunoResearch Labs	Cat#: #115-165-166; RRID:AB_2338692
Experimental Models: Organisms/Strains
Ir40a-LexA	[25]	N/A
Gr28b.d-Gal4	[54]	N/A
UAS-myr:GFP (P[10UAS-IVS-myr::GFP]attP1)	[55]	N/A
LexAop-mRFP	Bloomington Stock Center	Stock# 29956; RRID:BDSC_29956
Ir21a-T2A-Gal4	This paper	N/A
Ir68a-T2A-Gal4	This paper	N/A
yw, 3XP3-EGFP, Vasa-Cas9 attp18	Kate Koles, Rodal Lab Brandeis University	N/A
Oligonucleotides
gRNA against Ir21a intronic region: GCGCGTGAGTATTGCTTAAT	This paper	N/A
gRNA against Ir68a intronic region: GTGTGATATGAAAAGCATTC	This paper	N/A
Primer 1: Ir21a 5′ homology arm: atacgactcactatagggcgacccaccggtCGATATG TCATATTATTGGGTAGCTCTGGT	This paper	N/A
Primer 2: Ir21a 5′ homology arm: ccgaaaaccgcttctgacctggggcggccgcAA GCAATACTCACGCGCAT	This paper	N/A
Primer 3: Ir21a 3′ homology arm: ccgaaaaccgcttctgacctgggggcgcgccAATAGG GATACGTTTTTGTAACAAATAATGCGCTT CACACAGGAG	This paper	N/A
Primer 4: Ir21a 3′ homology arm: aagggaacctccccactagtggtaccCTCAAATGA AGCGCCGATCGG	This paper	N/A
Primer 5: Ir68a 5′ homology arm: atacgactcactatagggcgacccaccggtGGAAC CTCTTTGCCAGTTGCC	This paper	N/A
Primer 6: Ir68a 5′ homology arm: ccgaaaaccgcttctgacctggggcggccgcTGCTT TTCATATCACACTAGATTATTTTG	This paper	N/A
Primer 7: Ir68a 3′ homology arm: ccgaaaaccgcttctgacctgggggcgcgccTTCC GGCGACTTAATGGCTTTGT	This paper	N/A
Primer 8: Ir68a 3′ homology arm: aagggaacctccccactagtggtaccCTTCTAAAG AGATGGCCAAGCAAAAGC	This paper	N/A
Recombinant DNA
pCFD3-dU6:3gRNA	[56]	N/A
pGEM (phase 0)	[57]	N/A
Deposited Data
The Full Adult Fly Brain ssTEM dataset	[30]	https://temca2data.org/
FAFB manual neuronal reconstructions	This paper [30, 33, 51, 58, 59],	https://fafb.catmaid.virtualflybrain.org/ Neurons can be retrieved by their SKID (skeleton identifier).
Data S1 Skeletons and meshes	This paper [30, 33, 51, 58, 59],	https://doi.org/10.5281/zenodo.3879033
FlyCircuit 1.2 database of Drosophila brain neurons	[60]	http://www.flycircuit.tw/
Fly Light GAL4/LexA collection	[26]	http://flweb.janelia.org/cgi-bin/flew.cgi
Software and Algorithms
CATMAID	[61, 62]	https://github.com/catmaid/CATMAID
natverse, including the nat, nat.flybrains, elmr, tracerutils, flycircuit, and rcatmaid packages	[63]	https://github.com/natverse/
NBLAST algorithm and R package	[31, 63]	https://github.com/natverse/nat.nblast
Pymaid, python code for interacting with CATMAID	[33]	https://github.com/schlegelp/pymaid
FAFBseg, python code for working with the partial autosegmentation of FAFB from [53]	[33]	https://github.com/flyconnectome/fafbseg-py
CATMAID-to-Blender	[64]	https://github.com/schlegelp/CATMAID-to-Blender
rmushroom package	[30]	https://github.com/flyconnectome/rmushroom