REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-GFP	Abcam	RRID: AB_300798
Alexa Fluor Goat anti-mouse	Invitrogen	RRID: AB_2633275
nc82	Developmental Studies Hybridoma Bank	RRID: AB_2314866
Chemicals, Peptides, and Recombinant Proteins
Sucrose	Sigma-Aldrich	Cat# S0389
Mineral Oil	Sigma-Aldrich	Cat#M5904
4-methylcyclohexanol (98%)	Sigma-Aldrich	Cat#218405
3-octanol (99%)	Sigma-Aldrich	Cat#153095
Retinal	Sigma-Aldrich	Cat#R2500
DEET	Sigma-Aldrich	Cat#36542
Xylose	Sigma-Aldrich	Cat#X1500
Normal goat serum	Invitrogen	Cat#16210064
VectaShield	VectorLabs	SKU#H-1700-10
Deposited Data
Greyscale EM Data and raw neuronal traces	[4]	https://catmaid.virtualflybrain.org
Skeletonized traces – will be deposited on NeuroMorpho.Org and VirtualFlyBrain.org	This paper	https://v2.virtualflybrain.org/; http://neuromorpho.org/
Experimental Models: Organisms/Strains
Drosophila: Canton-S	Waddell Lab	N/A
Drosophila: R58E02-GAL4	FlyLight, Bloomington Drosophila Stock Center (BDSC)	RRID: BDSC_41347 [20, 28]
Drosophila: MB504B-GAL4	FlyLight, BDSC	RRID: BDSC_68329 [2, 20, 22]
Drosophila: MB315C-GAL4	FlyLight, BDSC	RRID: BDSC_68316 [2, 20, 22]
Drosophila: MB320C-GAL4	FlyLight, BDSC	RRID: BDSC_68253 [2, 20, 22]
Drosophila: 0273-GAL4	InSITE collection	Waddell lab [19, 30]
Drosophila: MB077B-GAL4	FlyLight, BDSC	RRID: BDSC_68283 [2, 20, 22]
Drosophila: VT006202-GAL4	Vienna Tiles Project	RRID: BDSC_72445 [8, 21]
Drosophila: 0104-GAL4	InSITE collection	RRID: BDSC_62639 [19, 30]
Drosophila: 0804-GAL4	InSITE collection	RRID: BDSC_6325 [9, 19]
R48B04-GAL80; 0104-GAL4	FlyLight/ InSITE	Waddell lab [12]
Drosophila: GMR88F08-GAL4	FlyLight, BDSC	RRID: BDSC_47982
Drosophila: GMR88E01-GAL4	FlyLight, BDSC	RRID: BDSC_48397
Drosophila: GMR66C08-GAL4	FlyLight, BDSC	RRID: BDSC 49412 [11, 20]
Drosophila: SS46348-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS28305-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS27959-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS52050-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS45245-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS45251-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS52046-GAL4	Ito/Rubin labs	unpublished data
Drosophila: S49970-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS45221-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS47568-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS49941-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS39616-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS27580-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS28308-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS21077-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS45222-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS42968-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS46613-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS35902-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS51316-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS31522-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS21206-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS31827-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS39070-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS25028-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS24588-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS27978-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS46337-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS32768-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS36651-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS46596-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS42983-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS24252-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS21208-GAL4	Ito/Rubin labs	unpublished data
Drosophila: SS32151-GAL4	Ito/Rubin labs	unpublished data
Drosophila: LH991-GAL4	Jefferis Lab	[24]
Drosophila: LH2241-GAL4	Jefferis Lab	[24]
Drosophila: LH989-GAL4	Jefferis Lab	[24]
Drosophila: LH1395-GAL4	Jefferis Lab	[24]
Drosophila: LH2278-GAL4	Jefferis Lab	[24]
Drosophila: LH1538-GAL4	Jefferis Lab	[24]
Drosophila: LH1539-GAL4	Jefferis Lab	[24]
Drosophila: LH1396-GAL4	Jefferis Lab	[24]
Drosophila: UAS-Shibirets1	BDSC	RRID: BDSC_44222 [27]
Drosophila: UAS-CsChrimson.mVenus	BDSC	RRID: BDSC_55135 [23]; RRID: BDSC_55136
Software and Algorithms
Blender 3D v.2.79	Blender Online Community	https://www.blender.org/
Natverse	[39]	https://github.com/natverse/nat/
Dendroextras	[40]	https://github.com/jefferis/dendroextras/
R	R Development Core Team	http://www.R-project.org/
Rcatmaid	Jefferis and Cardona labs	https://github.com/natverse/rcatmaid
CATMAID	[41, 42]	https://catmaid.readthedocs.io/en/stable/index.html#
Python	Python Software Foundation	https://www.python.org/
PyMaid	Philipp Schlegel	https://github.com/schlegelp/PyMaid
Dendrogram code	Markus Pleijzier	https://github.com/markuspleijzier/AdultEM/tree/master/Dendrogram_code
Graphviz	[43]	https://www.graphviz.org
NetworkX	[44]	https://networkx.github.io/
Dendextend	[40]	https://github.com/jefferis/dendroextras
Fiji	NIH [45]	http://fiji.sc/
GraphPad Prism 6	GraphPad Software, La Jolla, CA	https://www.graphpad.com/scientific-software/prism/
Adobe Illustrator CC	Adobe Systems, San Jose, CA	https://www.adobe.com/uk/products/illustrator.html
Vegan	Vegandevs - Jari Oksanen	https://github.com/vegandevs/vegan
SciPy	[46]	https://www.scipy.org/
Nbclust	[47]	https://rdrr.io/cran/NbClust/man/NbClust.html