Fig. 3.
N-JMJD5 binds to dual Ser2p-CTD of Pol II with high affinity. (A) MicroScale Thermophoresis measurement of the binding of MBP-N-JMJD5 to native CTD peptide. (B) The binding of MBP-N-JMJD5 to dual Ser5p-CTD peptide. (C) The binding of MBP-N-JMJD5 to single Ser2p-CTD peptide. (D) The binding of MBP-N-JMJD5 to dual Ser2p-CTD peptide. (E) The binding of MBP-N-JMJD5 to dual Ser2p-CTD peptide with Ser5p within the first repeat. (F) The binding of MBP-N-JMJD5 to dual Ser2p-CTD peptide with Ser5p within the second repeat.