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. 2020 Aug 3;117(33):20117–20126. doi: 10.1073/pnas.2003900117

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Fig. 1. — Clinical immunophenotypic characteristics and morphological features of distinct leukemic cell populations in t(8;21) AML patients. (A) Representative clinical flow cytometry data showing the distribution of CD34⁺ myeloblasts by antigen CD34 and CD117 in four t(8;21) AML patients at diagnosis (Upper) and four AML patients with other FAB subtypes (except for M3) at diagnosis (Lower). (B, Upper) Representative Wright Giemsa-stained cytospin preparations of isolated CD34⁺CD117^dim, CD34⁺CD117^bri, and AM cell populations from the BM of t(8;21) AML patients. The AM population was isolated using the marker combination of CD34⁻CD117⁻HLA-DR⁻CD15⁺CD11b⁻. (B, Lower) Differential counts of the isolated cell populations (mean ± SD; n = 4), including CD34⁺CD117^dim, CD34⁺CD117^bri, and AM populations.