Fig. 2.

Effect of a denaturing agent on the temperature-driven gating of TRPM8 channels. Representative recordings of macroscopic currents of TRPM8 and TRPM8ΔCT36 evoked by voltage pulses ranging between −140 and 420 mV (A–D) in standard recording solution and (E–H) in the presence of 3M urea at the temperature indicated. For the sake of clarity, only one of every two current records is shown in A–H. (I–L) Average G–V relations in the absence (open black circles) and in the presence of urea (blue open circles are data at 10 °C; red open circles are data at 25 °C) obtained from normalized tail currents, Solid lines are Boltzmann fits to Eq. S1 (SI Appendix, Eq. S1). Fit parameters are summarized in SI Appendix, Table S2. Detailed voltage protocols are given in SI Appendix, Table S3. Error bars are SE, n = 4 to 6.