Skip to main content
. 2020 Aug 3;117(33):20298–20304. doi: 10.1073/pnas.2004303117

Fig. 3.

Fig. 3.

Temperature-dependent changes in the solute-inaccessible aqueous volume of TRPM8 channels. Representative recordings of macroscopic currents of (A and B) TRPM8 and (C and D) TRPM8ΔCT36 channels in the presence of internal 2 M sucrose at 10 and 25 °C evoked by voltage pulses ranging between −140 and 420 mV in 5-mV increments. For the sake of clarity, only one of every two current records is shown in AD. (EH) Average G–V relationships obtained from normalized tail currents. Solid lines are Boltzmann fits to Eq. S1 (SI Appendix, Eq. S1). Blue open circles are data obtained at 10 °C, and red open circles are data obtained at 25 °C. Fit parameters are summarized in SI Appendix, Table S2. Detailed voltage protocols are given in SI Appendix, Table S3. Error bars are SE, n = 4 to 7.