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. 2020 Jul 31;117(33):20316–20324. doi: 10.1073/pnas.2007245117

Table 1.

Generation of higher-order cslc mutants

Higher-order mutant line Female parent Male parent
Double mutants
cslc45-1 cslc5-1 cslc4-1
cslc46-1 cslc6-1 cslc4-1
cslc45-2 cslc5-1 cslc4-3
cslc46-2 cslc6-1 cslc4-3
cslc48 cslc4-3 cslc8
cslc68 cslc8 cslc6-1
cslc612 cslc6-1 cslc12-2
Triple mutants
cslc456-1 cslc45-1 cslc46-1
cslc456-2 cslc45-2 cslc46-2
cslc458 cslc48 cslc45-2
cslc468 cslc46-2 cslc48
cslc4612 cslc612 cslc46-2
cslc5612 cslc56 cslc612
Quadruple mutants
cslc4568 cslc458 cslc468
cslc45612 cslc4612 cslc5612
Quintuple mutant
cslc456812 cslc45612 cslc4568

The table shows the parental lines used to produce the higher-order mutants used in this work. Two cslc456 triple mutants, cslc456-1 and cslc456-2, were generated with the cslc4-1 and cslc4-3 alleles, respectively. The cslc quadruple and quintuple mutants were generated with the cslc4-3 allele.