Table 2.

Quantification of isoprimeverose (IP) after Driselase digestion of AIR of various Arabidopsis mutants

Genotype	IP, µg/mg AIR	Relative abundance of IP peak, %
Col-0	8.23 ± 1.33	100
xxt1 xxt2	n.d.	n.d.
clsc456812	n.d.	n.d.
cslc45612	1.00 ± 0.61*	12.2
cslc4568	1.02 ± 0.53*	12.4
cslc456-2	1.87 ± 0.55*	22.7
cslc456-1	3.40 ± 0.96*	41.3
clsc12-2	9.60 ± 2.38	116.6
cslc8-1	10.11 ± 2.11	122.8
cslc6-1	8.18 ± 0.87	99.4
clsc5-1	7.54 ± 1.25	91.5
cslc4-3	8.09 ± 2.47	98.3

The IP content was determined using HPAEC (Fig. 4). IP quantification (µg/mg AIR) was determined using maltose as an internal standard. The IP values were obtained from three biological replicates (n = 3, ±SD). Asterisks indicate statistically significant differences from Col-0, (Student’s t test, P < 0.05). The relative abundance of the IP peak in the samples was calculated with respect to Col-0. n.d., not detected.